(BT05454) PLANT BIOTECHNOLOGY

UNIT I TISSUE CULTURE
Introduction to cell and tissue culture; Tissue culture media (composition,
preparation); Initiation and maintenance of callus and cell suspension culture,
organogenesis; Protoplast isolation culture and fusion;
UNIT II TISSUE CULTURE APPLICATIONS I
Production of haploids, Somaclonal variations, Germplasm conservation
(Cryopreservation);
UNIT III TISSUE CULTURE APPLICATIONS II
Production of secondary metabolites from plant cell cultures; Processes for
enhancing the production of secondary metabolites. Technology of plant cell culture
for production of chemicals; Bioreactors systems and models for mass cultivation
of plant cells;
UNIT IV PLANT TRANSFORMATION TECHNOLOGY
Agrobacterium mediated gene transfer; Agrobacterium based vectors, viral vectors
and
their application. Direct gene transfer methods; chemical methods, electroporation,
microinjection, particle bombardment.
UNIT V PLANT GENETIC ENGINEERING FOR PRODUCTIVITY AND
PERFORMANCE I (BIOTIC STRESS)
Herbicide resistance, Insect resistance, Disease resistance, virus resistance,.
UNIT VI PLANT GENETIC ENGINEERING FOR PRODUCTIVITY AND
PERFORMANCE II (ABIOTIC STRESS)
Abiotic stress tolerance ;Drought, temperature, salt .
UNIT VII MOLECULAR FARMING & INDUSTRIAL PRODUCTS
Application of Plant biotechnology for the production of quality oil, Industrial enzymes,
Antigens (edible vaccine) and plantibodies.
UNIT VIII METABOLIC ENGINEERING
Metabolic engineering for plant secondary metabolites.
TEXT BOOKS:
1. Roberta Smith, Plant Tissue Culture: Techniques and Experiments. 2nd ed.,
Academic Press, 2000.
2. Bhojwani, S.S. and Rajdan, Plant Tissue Culture: Theory and Practice. 2004
REFERENCES:
1. Roberta Smith, Plant Tissue Culture: Techniques and Experiments, Academic
Press; 2nd ed, 2000.
2. Crispeels, M.J. and Sadava, D.E., Plants, Genes and Crop Biotechnology,
Jones and Bartlett Publishers (2nd Edition), 2003.
3. Bhowjwani, S.S., Plant Tissue Culture: Application and Limitations. Amsterdam,
Elsevier,1990.
4. Charles Cunningham and Andrew J.R. Porter, Recombinant Proteins from
Plants: Production and Isolation of Clinically
Useful Compounds (Methods in Biotechnology), Humana Press, 1997.
5. Bernard R. Glick and John E. Thompson, Methods in Plant Molecular Biology
and Biotechnology, CRC Press, 1993.
6. I. Potrykus and G. Spangenberg, , Gene Transfer to Plants (Springer Lab
Manual), Springer Verlag, 1997.
7. Peter M. Gresshoff, Plant Genome Analysis: Current Topics in Plant Molecular
Biology. CRC Press, 1994.
8. John Hammond, Peter McGarvey, Vidadi Yusibov, Plant Biotechnology: New
Products and Applications, Springer Verlag, 1999.