(BT05413) MOLECULAR BIOLOGY

Unit I: Structure of DNA
Detailed structure of DNA, variation from Watson & Crick model, Z - DNA, A & B
DNA, Denaturation & melting curves.
UNIT-II: DNA Replication - I
Models of DNA replication: semi conservative Mechanism of DNA replication in
E.coli (bi- directional). Mitochondrial (D-loop), Viral DNA (Rolling circle), Single
stranded- DNA phages (M13, 174),
Unit – III : DNA Replication- II
Eukaryotic telomeres and its replication Inhibitors of DNA Replication. Enzymes
involved in replication, step by step process.
Unit IV: RNA Structure and Biosynthesis
m-RNA, r-RNA, t-RNA structures, Transcription apparatus, RNA polymerases and
proteins involved in transcription (initiation, elongation and termination steps)
UNIT V : Post transcriptional processing
Post transcriptional processing of RNA ‘s t-RNA, r-RNA, m- RNA splicing. Inhibitors
of transcription.
Unit VI: Protein Biosynthesis
The genetic code and Wobble Hypothesis, Codon usage, Protein synthesis In
Prokaryotes.
UNIT VII : Protein synthesis in eukaryotes
Eukaryotic Protein synthesis, differences between prokaryotic and eukaryotic protein
synthesis,Post translation modifications. Inhibitors of protein synthesis
Unit VII : Mutagenesis
Mutations, spontaneous, induced, lethal, mutagens their types and actions,
classifications of mutations, characters of mutations and applications.
Site - directed mutagenesis and reverse genetics. DNA damage and repair
mechanisms. Mutagenicity testing using microbial systems, Ames TEST.
Unit – VIII Genetic Recombination In Bacteria
Transfer of Characters, Genetic Recombination, Gene Transfer mechanisms ,
Plasmids,
Phase Crosses, Insertion of phase chromosomes
TEXT BOOKS
1. Molecular Biology, David Friefeldur, Norasa Publishing Home
2. Benjamin Lewin - Gene – VIII, 2002
References:
1. Lodish, H., Berk A., Zipursky, S.L. Matsudaria, P. Baltimore, D. and Darnell,
J.2003. Molecular Cell f Biology, Media connected, W.H. Freeman and
Company.
2. Bio Chemistry and Molecular biology by William H. Ellioff. Oxford Publications
3. Cell and Molecular biology by Philip Sheeler Donald E.Bianchi Wiley Publishers.
4. Cell and Molecular Biology 1996. De Robertis E.D.Pi and De Robertis E.M.F.
B I Waverly Pvt. Ltd., New Delhi.