(BT05383) METABOLIC ENGINEERING

UNIT I : Introduction
Identification of metabolic regulation is a key point in metabolic engineering.
Basic concepts of Metabolic Engineering – Overview of cellular metabolism –
Different models for cellular reactions, induction – Jacob Monod model and its
regulation, Differential regulation by isoenzymes, Feed back regulation.
UNIT II : Synthesis of Primary Metabolites
Amino acid synthesis pathways and its regulation at enzyme level and whole cell
level, Alteration of feed back regulation, Limiting accumulation of endproducts.
UNIT III : Biosynthesis of Secondary Metabolites
Regulation of secondary metabolite pathways, precursor effects, prophase,
idiophase relationship, Catabolite regulation by passing control of secondary
metabolism, producers of secondary metabolites, applications of secondary
metabolites.
UNIT IV : Bioconversions
Applications of Bioconversions, Factors affecting bioconversions, Specificity, Yields,
Co metabolism, Product inhibition, mixed or sequential bioconversions, Conversion
of insoluble substances.
UNIT V : Regulation of Enzyme Production
Strain selection, Genetic improvement of strains, Gene dosage, metabolic pathway
manipulations to improve fermentation, Feed back repression, Catabolite
Repression, optimization and control of metabolic activities. The modification of
existing - or the introduction of entirely new - metabolic pathways
UNIT VI : Metabolic flux
Integration of anabolism and catabolism, metabolic flux distribution analysisbioprocess,
material balance, kinetic types, equilibrium reaction. Experimental
determination method of flux distribution, Metabolic flux analysis and its applications,
Thermodynamics of cellular processes
UNIT VII : Metabolic engineering with Bioinformatics
Metabolic pathway modeling, Analysis of metabolic control and the structure
metabolic networks, Metabolic pathway synthesis algorithms,
UNIT Vlll : Applications of Metabolic Enginerring
Application in pharmaceuticals, chemical bioprocess, food technology, agriculture,
environmental bioremediation and biomass conversion.
TEXT BOOKS:
1. Wang.D.I.C Cooney C.L., Demain A.L., Dunnil.P. Humphrey A.E. Lilly M.D.,
Fermentation and Enzyme Technology, John Wiley and sons 1980.
2. Stanbury P.F., and Whitaker A., Principles of Fermentation Technology,
Pergamon Press, 1984.
References :
Zubay G., Biochemistry, Macmillan Publishers, 1989.
http://ocw.osaka-u.ac.jp/contents/19/ME040512.pdf For unit Vl & Vll
http://ocw.osaka-u.ac.jp/contents/19/ME040421.pdf For unit Vl
http://ocw.osaka-u.ac.jp/contents/19/ME040526.pdf For unit Vll
http://ocw.osaka-u.ac.jp/contents/19/ME040602.pdf For unit Vl & Vll
http://www.bioinfo.de/isb/gcb01/poster/hurlebaus.html
How will bioinformatics influence metabolic engineering? Biotechnol Bioeng. 1998
Apr 20-May 5;58(2-3):162-9. For unit Vll