(BT05114) CELL BIOLOGY AND MICROBIOLOGY

1. Units, Volume & Weight
1. Calibration of microscope
2. Identification of Animal, Plant & Bacterial cells.
3. Micrometry.
4. Blood cells, WBC differential count.
5. Differential centrifugation and isolation of Chloroplast & Mitochondria.
6. Sterilization techniques (lecture/demonstrations)
7. Preparation of culture media (a) Broth type of media (b) Solid media
8. Culturing of microorganisms: (a) Broth (b) Pure culture techniques: Streak
plate, pour plate.
9. Isolation and preservation of bacterial culture.
10. Identification of microorganisms (a) Staining technique (b) Biochemical
testing.
11. Antibiotic test - Disc diffusion method, minimum inhibitory concentration.
12. Microbiological examination of water.
13. Biochemical tests
IMVIC test
Catalase test
Coagulase test
Gelatinase test
Oxidase test.
14. Determination of bacterial growth by turbidometry / colorimetry.
15. Factors affecting the bacterial growth - effect of Temperature & pH.
TEXT BOOKS:
1. Microbiological and applications, Laboratory, manual in general microbiology
by Benson, Mc Graw Publications.
2. Laboratory manual in microbiology by P. Gunasekharan Newage international
Publishers.