(BT05041) ANIMAL CELL SCIENCE AND TECHNOLOGY

Unit I:
Structure and Organization of animal cell; Equipments and materials for animal cell
culture technology; Primary and established cell line cultures; Introduction to the
balanced salt solutions and simple growth medium,
Unit II:
Brief discussion on the chemical, physical and metabolic functions of different
constituents of culture medium. Role of carbon dioxide. Role of serum and
supplements; Serum & protein free defined media and their application.
Unit III:
Measurement of viability and cytotoxicity; Biology and characterization of the cultured
cells, measuring parameters of growth;
Unit IV:
Basic techniques of mammalian cell culture in vitro; disaggregation of tissue and
primary culture, maintenance of cell culture; cell separation.
Unit V:
Cell synchronization; Cell cloning and micromanipulation; Cell transformation;
Application of animal cell culture; Scaling-up of animal cell culture.
Unit VI:
Stem cell cultures, embryonic stem cells and their applications; Cell culture based
vaccines, Somatic cell genetics.
Unit VII:
Organ and histotypic cultures; Measurement of cell death; Apoptosis;
Unit VIII:
Three dimensional culture and tissue engineering.
TEXT BOOKS:
1. Culture of Animal Cells, (3rd Edition), F1. Ian Froshney. Wiley-Liss.
2. Animal Cell Culture – Practical Approach, Ed. John R.W. Masters, OXFORD.
REFERENCES
1. Cell Culture Lab Fax. Eds. M. Butler & M. Dawson, Bios Scientific Publications
Ltd., Oxford.
2. Animal Cell Culture Techniques. Ed. Martin Clynes, Springer.
3. Methods in Cell Biology, Vol. 57, Animal Cell Culture Methods. Ed. Jenni P.
Mather and David Barnes. Academic Press.
4. Cell Growth and Division: A Practical Approach. Ed. R. Basega, IRL Press.