(BT05081) BIOCHEMISTRY LABORATORY

1. Units, Volume & Weight measurements. Concentration units, pH
Measurement. Preparation of buffers,
2. Qualitative tests for carbohydrates. Estimation of Reducing sugars by the
Benedict’s method.
3. Qualitative tests for Amino Acids. Quantitative method for Amino Acids,
Ninhydrin method
4. Protein estimation by Biuret / Folin’s / Bradford method.
5. Extraction of lipids. Saponification of Fats.
6. Estimation of cholesterol.
8. Estimation of Nucleic Acids, Precipitation by sodium sulphate, Test for ribose
and deoxyribose sugar.
9. Extraction of Caffeine from tea leaves.
10. Hydrolysis of ester using Papain.
11. Preparation of ∝-d Glucopyranose penta acetate from Glucose.`
TEXT BOOK:
1. Laboratory manaual in biochemistry by J.Jayaraman New age International
Publications.