Friday, November 30, 2007

(BT05240) ENZYME ENGINEERING

Unit I: Applications of Enzymes
Classification of Enzymes; Purification and characterization of enzymes from natural
sources Comparison of chemical and enzyme catalysis.
Unit- II : Isolation of Enzymes:
Extraction and Purification of Crude Enzyme extracts from plant, animal and microbial
sources-some case studies; methods of characterization of enzymes; development
of enzymatic assays.
Unit III: Mechanisms and Kinetics of Enzyme Action
Mechanisms of Enzyme Action; Concept of active site and energetics of enzyme
substrate complex formation; Specificity of enzyme action; Kinetics of single substrate
reactions; turnover number; estimation of Michaelis-Menton parameters. Importance
of K
M
, Multi-substrate reaction mechanisms and kinetics.
UNIT IV : Enzyme Inhibition
Types of Inhibition- kinetic models; Substrate and Product Inhibition; Allosteric
regulation of enzymes; Deactivation kinetics.
Unit V: Enzyme Immobilization
Physical and Chemical techniques for enzyme Immobilization - adsorption. matrix
entrapment, encapsulation. cross-linking. covalent binding - examples; Advantages
and disadvantages of different Immobilization techniques. overview of applications
of immobilized enzyme systems.
Unit VI: Mass Transfer Effects In Immobilized Enzyme Systems and Design of
Enzyme Reactors
Analysis of Film and Pore Diffusion Effects on kinetics of Immobilized Enzyme
Reactions; Formulation of dimensionless groups and calculation of Effectiveness
Factors
Unit VII: Enzyme Reactors:
Design of Immobilized Enzyme Reactors-Packed- bed, Fluidized-bed Membrane
reactors; Bioconversion calculations in free- enzyme CSTRs and immobilized
enzyme reactors.
Unit VII: Enzyme Biosensors
Applications of enzymes in analysis; Design of enzyme electrodes and their
applications as biosensors in industry, health care and environment.
TEXT BOOKS:
1 Enzymes by Trevor palmer , East west Press
2. Biochemical engineering fundamentals, second edition. James E Bailey, David
F., Ollis, Mc Graw Hill Intl. Edition.
REFERENCES:
1. Basic Biotechnology, Second Edition, by Colin Ratledge and Bjorm
Kristiansen, Cambridge University Press.
2. Biochemical Engineering by James Lee.

2 comments:

Unknown said...

give information on bio-informatics and also the reference books for that subject

Unknown said...

David Mount is the best book for
preparing...n All the ebooks are avail in internet...