(BT05414) MOLECULAR BIOLOGY AND GENETIC ENGINEERING LAB

1. Isolation of Plant and Bacterial Genomic DNA and Plasmid DNA.
2. Agarose Gel Electrophoresis.
3. Restriction Enzyme digestion.
4. Demonstration Chemical mutagenesis.
5. Isolation and visualization of plasmid on Agarose gel.
6. Restriction mapping and ligation.
7. Transformation, screening for recombinants.
8. Characterization of secondary metabolites by Polyacrylamide gel
Electrophoresis. Silver staining of protein gels.
9. Blotting Techniques.
10. Expression of Beta – galactosidase and assay.
11. Cloning of DNA into plasmid vector.
REFERENCES:
1. Old RW and Primrose SB, Principles of gene manipulation, Blackwell
Scientific Publications, 1992.
2. Freifelder D, Molecular Biology, Jones and Bartlett Publishers inc. 1987.