Friday, November 30, 2007

(BT05181) DOWNSTREAM PROCESSING

Unit I: Role Of Downstream Processing In Biotechnology
Role and importance of downstream processing in biotechnological processes.
Problems and requirements of bioproduct purification. Economics of downstream
processing in Biotechnology, cost-cutting strategies, characteristics of biological
mixtures, process design criteria for various classes of bioproducts (high volume,
low value products and low volume, high Value products)
UNIT-II:
Physico-chemical basis of bio-separation processes. Recent development in product
isolation (for ex. one step purification, reverse Micro cellular extraction on line
membrane separation).
Unit III: Primary Separation And Recover Process
Cell disruption methods for intracellular products, removal of insoluble, biomass
(and particulate debris) separation techniques, flocculation and sedimentation,
centrifugation and filtration methods.
Unit IV: Membrane separations :
Membrane-based separations (micro and ultrafiltration), theory, design and
configuration of membrane separation equipment applications,
Unit V: Enrichment Operations
precipitation methods (with salts, organic solvents, and polymers, extractive
separations, aqueous two-phase extraction, supercritical extraction), in situ product
removal, integrated bioprocessing.
Unit VI: Electrophoresis :
Elecrtrophoresis of proteins and nucleic acids, 1D-2D Gels, Types of Electrophoretic
techniques (Capillary and Pulse field)
Unit VII Product Resolution / Fractionation
Chromatographic techniques- Paper, TLC, Adsorption, Ion exchange, Gel filtration,
affinity chromatographic separation processes, GC, HPLC, FPLC, Chromato
focusing electrophoretic separations.
Unit VIII: New and Emerging Technologies
Dialysis, Crystallization Pervaporation, super liquid extraction foam based
separation case study with examples for processing of Two Industrial Products
(Citric acid / Penicillin and Low volume high value product like recombinant proteins).
Text Books:
1. Wankat PC. Rate controlled separations, Elsevier, 1990.
2. Belter PA and Cussler E. Bioseparations, Wiley 1985.
References:
1. Product Recovery in Bioprocess Technology, BIOTOL.’ Series, VCH, 1990.
2. Asenjo J.M. Separation processes in Biotechnology, 1993, Marcel Dekkere
Inc.
3. Bioseparations by Siva Shankar PHI publications

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