(BT05097)BIOPROCESS ENGINEERING LAB- I

1. Enzyme Isolation and Assay of Enzymatic, Activity
Extraction of commercially important enzymes from natural source Development
of enzyme assays; quantification of enzyme activity and specific activity.
2. Enzyme Kinetics
Estimation of Michaelis Menten parameters, Effect of pH and temperature on enzyme
activity, kinetics of inhibition.
3. Immobilized Enzyme Reactions
Techniques of enzyme immobilization - matrix entrapment, ionic and cross linking;
column packing; analysis of mass transfer effects on kinetics of immobilized enzyme
reactions; bioconversion studies with immobilized - enzyme packed - bed
reactors.
4. Microbial Culture Studies:
Culturing of different types of microorganisms (bacteria, yeast, fungi) used in the
production of commercially important products; Formulation of simple and complex
culture media; Estimation of biomass (dry weight); substrate and product analysis;
Study of Growth substrate utilization and product formation kinetics in shake - flask
cultures.