(BT05319) INSTRUMENTAL METHODS OF ANALYSIS

Unit I: Introduction
Types of Analytical Methods – Instruments for Analysis – Uncertainties in
Instrumental measurements – Sensitivity and detection limit for instruments.
Unit II: Microscopy
Bright field, Dark field, Fluorescent, Phase contrast, confocal microscopy, SEM &
TEM Microscopy, Flow Cytometry.
Unit IV: Spectroscopy-I
General principles – Radiation, energy and atomic structure- types of spectra and
their biochemical usefulness – basic laws of light absorption. Electromagnetic
radiation & Spectrum, Beer – Lambert’s Law and apparent deviations; UV - VIS
Spectrophotometer,
Unit V: Spectroscopy-II
Spectrofluorimetry, Atomic absorption & Atomic emission spectroscopy, Cirular
Dichroism (CD)- principles, instrumentation and applications.
Unit VI: Spectroscopy-III
Infra Red Spectroscopy. Mass spectroscopy-Introduction, analysis, applications in
biology
Unit VII: NMR
High resolution NMR –Chemical shift-Spin-spin coupling Frequency lock- double
resonance-applications of proton NMR-quantitative analysis-qualitative analysis,
application of NMR in biology and study of macromolecules
Unit VIII: Spectroscopy
ESR principles - instrumentation-applications
TEXT BOOKS:
1. A Biologist Guide to principles and techniques of practical Biochemistry. By
Keith Wilson, Kenneth H. Goulding 3rd ed. ELBS Series.
2. Skoog & West, Fundamentals of Analytical Chemistry, 1982
REFERENCES:
1. Vogel, Text Book of Quantitative Inorganic Analysis, 1990
2. Ewing, Instrumental Methods of Analysis, 1992
3. Hobert H Willard D. L. Merritt & J. R. J. A. Dean, Instrumental Methods of
Analysis, CBS Publishers & Distributors, 1992